@article{34f881547d65421586ad992864acfd39,
title = "Skim milk enhances the preservation of thawed - 80 °C bacterial stocks",
abstract = "The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.",
keywords = "Bacterial strain, Cryopreservation, Glycerol, Skim milk",
author = "Cody, {William L.} and Wilson, {James W.} and Hendrixson, {David R.} and McIver, {Kevin S.} and Hagman, {Kayla E.} and Ott, {C. M.} and Cheryl Nickerson and Schurr, {Michael J.}",
note = "Funding Information: This study was supported by NIH grant RO1AI50812 to M.J.S, NASA grants NAG 2-1378, NAG9-1350, and NCC 2-1362 to C.A.N., NIH grant RO1AI51332 to K.E.H., R01AI47928 to K.S.M., Distinguished Young Investigator Award from the President's Research Council of the University of Texas Southwestern Medical Center to D.R.H. We thank Dr. Duane Pierson and his lab at the NASA Johnson Space Center and the Department of Microbiology at the University of Texas Southwestern Medical Center (UTSMC) at Dallas for their invaluable help in preserving our strains, and to Dr. Michael Norgard and UTSMC for hosting the Schurr laboratory following the hurricane. In addition, we thank Dr. Margaret Park for her help during the challenging recovery effort of strains at Tulane.",
year = "2008",
month = sep,
doi = "10.1016/j.mimet.2008.05.006",
language = "English (US)",
volume = "75",
pages = "135--138",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",
}