TY - JOUR
T1 - Substance P receptor expression and cellular responses to substance P in prenatal rat spinal cord cells
AU - St. John, Paul A.
AU - Ludwig, Christopher P.
AU - Lai, Josephine
N1 - Funding Information: We thank Sherry Stephens, Linda Nunan, and Melissa Langston for expert technical assistance. We are grateful to JamesK rause for the cDNA probes for the rat SPR and substance K -tor. We thank Janis Burt for ~ccessto the scanning analysis equipment. We also thank Chris McGhee for performing the densitometry an the autoradiograms. C.P.L. and C. McGhee were supported in part by the UA Undergraduate Biology Research Program This work was supported by grants to P.A.St.J. from NSF (BNS 8808506), NIH (NS296!57), the Arizona Disease Control Research Commission (#82-93111,a nd the University of i4rizuna Foundation, and to J.L. from the American Heart Association, Arizona Affiliate (#IG-2-44-91). The current address of C.P.L. is Neuroscience Program, Washington llniversity, St. Louis, MO.
PY - 1997
Y1 - 1997
N2 - Substance Preceptors (SPRs) are expressed by prenatal rat spinal cord neurons and glial cells early in their differentiation, and SPRs may mediate developmental influences in the developing spinal cord. In order to understand better early SPR expression, we quantified SPR mRNA in the rat spinal cord during prenatal development using a cDNA probe for the rat SPR in nuclease protection assays. SPR mRNA was present in the rat spinal cord at E14, the earliest stage examined, and the presence of specific binding sites for radiolabeled SP suggested that SPRs were expressed at the protein level as well. Comparisons of samples from rats at different prenatal ages showed that the relative abundance of SPR mRNA declined by about 75% from E14 through the remainder of prenatal development. Assays of the hydrolysis of phosphatidyl inositol performed on prenatal spinal cord cells in culture revealed that SP caused a small but significant stimulation. These results show that expression of SPRs is an early molecular event in the development of the rat spinal cord in vivo and that SPRs on young spinal cord cells can mediate functional responses at early developmental stages.
AB - Substance Preceptors (SPRs) are expressed by prenatal rat spinal cord neurons and glial cells early in their differentiation, and SPRs may mediate developmental influences in the developing spinal cord. In order to understand better early SPR expression, we quantified SPR mRNA in the rat spinal cord during prenatal development using a cDNA probe for the rat SPR in nuclease protection assays. SPR mRNA was present in the rat spinal cord at E14, the earliest stage examined, and the presence of specific binding sites for radiolabeled SP suggested that SPRs were expressed at the protein level as well. Comparisons of samples from rats at different prenatal ages showed that the relative abundance of SPR mRNA declined by about 75% from E14 through the remainder of prenatal development. Assays of the hydrolysis of phosphatidyl inositol performed on prenatal spinal cord cells in culture revealed that SP caused a small but significant stimulation. These results show that expression of SPRs is an early molecular event in the development of the rat spinal cord in vivo and that SPRs on young spinal cord cells can mediate functional responses at early developmental stages.
UR - http://www.scopus.com/inward/record.url?scp=0030878491&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030878491&partnerID=8YFLogxK
U2 - 10.3109/10799899709039150
DO - 10.3109/10799899709039150
M3 - Article
C2 - 9220368
SN - 1079-9893
VL - 17
SP - 569
EP - 583
JO - Journal of Receptor and Signal Transduction Research
JF - Journal of Receptor and Signal Transduction Research
IS - 4
ER -