@inbook{6c3e86786cdf4c73a11ba9a6759aca95,
title = "Transient protein expression by agroinfiltration in lettuce",
abstract = "Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed “deconstructed” viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.",
keywords = "Agrobacterium tumefaciens, Agroinfiltration, Antibodies, Biotechnology, Large-scale production, Lettuce, Plant biology, Plant-made pharmaceutics, Recombinant protein, Transient expression, Vaccines",
author = "Qiang Chen and Matthew Dent and Jonathan Hurtado and Jake Stahnke and Alyssa McNulty and Kahlin Leuzinger and Huafang Lai",
year = "2016",
month = may,
day = "1",
doi = "10.1007/978-1-4939-3289-4_4",
language = "English (US)",
volume = "1385",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "55--67",
booktitle = "Methods in Molecular Biology",
}