TY - JOUR
T1 - Two new endophytic species enrich the Coniochaeta endophytica/C. Prunicola clade
T2 - Coniochaeta lutea sp. nov. And C. palaoa sp. nov.
AU - Arnold, A. Elizabeth
AU - Harrington, Alison H.
AU - U'Ren, Jana M.
AU - Oita, Shuzo
AU - Inderbitzin, Patrik
N1 - Funding Information: We thank the School of Plant Sciences, the Department of Ecology and Evolutionary Biology, and the College of Agriculture and Life Sciences at the University of Arizona for supporting this study. We thank the National Science Foundation for supporting the studies that led to the isolation of Coniochaeta endophytes from diverse hosts (to AEA, NSF DEB-1045766, DEB-1010675, DEB-1541496), and for supporting AHH via a Graduate Research Fellowship. We acknowledge NIFA for support to AEA and colleagues (ARZT-1361340-H25-242) and to the Robert L. Gilbertson Mycological Herbarium (ARZT-1259370-S25-200). We thank Indigo Agriculture, Inc. for support relevant to the species description. We thank François Lutzoni and Jolanta Miadlikowska for co-leading field expeditions in which several of the focal strains were isolated, and we are grateful to local collaborators in each location. We are grateful to J. Carey, I. Kline, N. Yang, and C. Plecki for assistance in the laboratory, and especially to M. Lee for project coordination and logistical support. The authors declare no conflict of interest in presenting this work. Publisher Copyright: © 2021 W. Szafer Institute of Botany, Polish Academy of Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC BY 4.0 (http://creativecommons.org/licenses/by/4.0/)
PY - 2021
Y1 - 2021
N2 - Coniochaeta (Coniochaetaceae, Ascomycota) is a diverse genus that includes a striking richness of undescribed species with endophytic lifestyles, especially in temperate and boreal plants and lichens. These endophytes frequently represent undescribed species that can clarify evolutionary relationships and trait evolution within clades of previously classified fungi. Here we extend the geographic, taxonomic, and host sampling presented in a previous analysis of the clade containing Coniochaeta endophytica, a recently described species occurring as an endophyte from North America; and C. prunicola, associated with necroses of stonefruit trees in South Africa. Our multi-locus analysis and examination of metadata for endophyte strains housed in the Robert L. Gilbertson Mycological Herbarium at the University of Arizona (ARIZ) (1) expands the geographic range of C. endophytica across a wider range of the USA than recognized previously; (2) shows that the ex-type of C. prunicola (CBS 120875) forms a well-supported clade with endophytes of native hosts in North Carolina and Michigan, USA; (3) reveals that the ex-paratype for C. prunicola (CBS 121445) forms a distinct clade with endophytes from North Carolina and Russia, is distinct morphologically from the other taxa considered here, and is described herein as Coniochaeta lutea; and (4) describes a new species, Coniochaeta palaoa, here identified as an endophyte of multiple plant lineages in the highlands and piedmont of North Carolina. Separation of CBS 120875 and CBS 121445 into C. prunicola sensu stricto and C. lutea is consistent with previously described genomic differences between these isolates, and morphological and functional differences among the four species (C. endophytica, C. prunicola, C. palaoa, and C. lutea) underscore the phylogenetic relationships described here. The resolving power of particular loci and the emerging perspective on the host- and geographic range of Coniochaeta and the C. endophytica / C. prunicola clade are discussed.
AB - Coniochaeta (Coniochaetaceae, Ascomycota) is a diverse genus that includes a striking richness of undescribed species with endophytic lifestyles, especially in temperate and boreal plants and lichens. These endophytes frequently represent undescribed species that can clarify evolutionary relationships and trait evolution within clades of previously classified fungi. Here we extend the geographic, taxonomic, and host sampling presented in a previous analysis of the clade containing Coniochaeta endophytica, a recently described species occurring as an endophyte from North America; and C. prunicola, associated with necroses of stonefruit trees in South Africa. Our multi-locus analysis and examination of metadata for endophyte strains housed in the Robert L. Gilbertson Mycological Herbarium at the University of Arizona (ARIZ) (1) expands the geographic range of C. endophytica across a wider range of the USA than recognized previously; (2) shows that the ex-type of C. prunicola (CBS 120875) forms a well-supported clade with endophytes of native hosts in North Carolina and Michigan, USA; (3) reveals that the ex-paratype for C. prunicola (CBS 121445) forms a distinct clade with endophytes from North Carolina and Russia, is distinct morphologically from the other taxa considered here, and is described herein as Coniochaeta lutea; and (4) describes a new species, Coniochaeta palaoa, here identified as an endophyte of multiple plant lineages in the highlands and piedmont of North Carolina. Separation of CBS 120875 and CBS 121445 into C. prunicola sensu stricto and C. lutea is consistent with previously described genomic differences between these isolates, and morphological and functional differences among the four species (C. endophytica, C. prunicola, C. palaoa, and C. lutea) underscore the phylogenetic relationships described here. The resolving power of particular loci and the emerging perspective on the host- and geographic range of Coniochaeta and the C. endophytica / C. prunicola clade are discussed.
KW - Actin
KW - Anamorph
KW - Barcode
KW - Biodiversity
KW - Endolichenic
KW - Lecythophora
UR - http://www.scopus.com/inward/record.url?scp=85113317214&partnerID=8YFLogxK
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U2 - 10.35535/PFSYST-2021-0006
DO - 10.35535/PFSYST-2021-0006
M3 - Article
SN - 1641-8190
VL - 66
SP - 66
EP - 78
JO - Plant and Fungal Systematics
JF - Plant and Fungal Systematics
IS - 1
ER -