TY - JOUR
T1 - USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components
AU - Zhao, Wei
AU - Qian, Chen
AU - Liu, Pinghua
AU - Li, Qingtian
AU - Li, Wenyuan
AU - Wang, Rong Fu
N1 - Funding Information: This work was in part supported by grants from the NCI, NIH (R01CA090327 and R01CA101795), Cancer Prevention and Research Institute of Texas (CPRIT), National Natural Science Foundation of China (No. 81572766), and Dottie and Jimmy C. Adair Myelodysplastic Syndrome Research and Treatment Fund. W.L. was supported in part by Xiangya Hospital, Xiangya School of Medicine, Central South University, China. We would like to thank Dr. Jana Burchfield for critical reading of this manuscript. Publisher Copyright: © 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Despite much progress in the comprehension of the complex process of somatic cell reprogramming, many questions regarding the molecular mechanism of regulation remain to be answered. At present, the knowledge on the negative regulation of reprogramming process is indeed poor in contrary to the identification of positive regulators. Here we report for the first time that ubiquitin-specific protease 26 negatively regulates somatic cell-reprogramming process by stabilizing chromobox (CBX)-containing proteins CBX4 and CBX6 of polycomb-repressive complex 1 through the removal of K48-linked polyubiquitination. Thus, accumulated CBX4 and CBX6 repress the expression of pluripotency genes, such as Sox2 and Nanog, through PRC1 complexes to ubiquitinate histone H2A at their promoters. In all, our findings have revealed an essential role for ubiquitin-specific protease 26 in cellular reprogramming through polycomb-repressive complex 1.
AB - Despite much progress in the comprehension of the complex process of somatic cell reprogramming, many questions regarding the molecular mechanism of regulation remain to be answered. At present, the knowledge on the negative regulation of reprogramming process is indeed poor in contrary to the identification of positive regulators. Here we report for the first time that ubiquitin-specific protease 26 negatively regulates somatic cell-reprogramming process by stabilizing chromobox (CBX)-containing proteins CBX4 and CBX6 of polycomb-repressive complex 1 through the removal of K48-linked polyubiquitination. Thus, accumulated CBX4 and CBX6 repress the expression of pluripotency genes, such as Sox2 and Nanog, through PRC1 complexes to ubiquitinate histone H2A at their promoters. In all, our findings have revealed an essential role for ubiquitin-specific protease 26 in cellular reprogramming through polycomb-repressive complex 1.
UR - https://www.scopus.com/pages/publications/85028089114
UR - https://www.scopus.com/pages/publications/85028089114#tab=citedBy
U2 - 10.1038/s41467-017-00301-4
DO - 10.1038/s41467-017-00301-4
M3 - Article
C2 - 28839133
SN - 2041-1723
VL - 8
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 349
ER -